Faculty Profile

Jie Zheng
ASSOCIATE PROFESSOR
Physiology and Membrane Biology
4145 Tupper Hall
Office 530-752-1241
Lab 530-752-8368
jzheng@ucdavis.edu
http://www.physiology.ucdavis.edu/zheng/index.html
[Picture of Jie Zheng]

Degrees:
1998 - PhD - Yale University - Physiology
1991 - MS - Beijing University - Biophysics
1988 - BS - Beijing University - Physiology and Biophysics

Awards:
University Scholarship, Yale University, 1992-1995
Guanghua Scholarship, Beijing University, 1988
Research Associate, Howard Hughes Medical Institute, 2000-2003
UCD Health Science Research Award, 2008
the Grass Foundation Travelling Scientist Award, 2009

Department and Center Affiliations:
Department of Physiology and Membrane Biology

Professional Societies:
Society for Neuroscience
Biophysical Society
Society of General Physiology
American Physiological Society
Editorial Board member, Journal of Ion Channels

Grad Group Affiliations and Specialties:
Molecular, Cellular, and Integrative Physiology
Non-DBS Grad Group(s) - Pharmacology and Toxicology

Publications:
Qu, Z., W. Cheng, Y. Cui, Y. Cui, and J. Zheng (2009) Human disease-causing mutations disrupt an N-C terminal interaction and channel function of bestrophin 1, Journal of Biological Chemistry, 284:16473-16481

Sun, P., J. Wang, W. Gu, W. Cheng, G.-Z. Jin, E. Friedman, J. Zheng, and X. Zhen (2009) PSD-95 regulates D1 dopamine receptor resensitization, but not receptor-mediated Gs-protein activation, Cell Research, 19:612-624

Qiu, S., X.-M. Zhang, J.-Y. Cao, W. Yang, Y.-G. Yan, L. Shan, J. Zheng, and J.-H. Luo (2009) An ER retention signal located in the extracellular amino-terminal domain of NR2A subunit of NMDA receptors, Journal of Biological Chemistry, 284:20285-20298

Liang, S.-S., F. Yang, C. Zhou, Y. Wang, S. Li, C.K. Sun, J.L. Puglisi, D. Bers, C. Sun, and J. Zheng (2009) Temperature-dependent activation of neurons by continuous near-infrared laser, Cell Biochemistry and Biophysics, 53(1), 33-42

Zheng, J., and K. Matulef (2009) Cyclic nucleotide-gated ion channels, In: Encyclopedia of Life Sciences (ELS), John Wiley & Sons, Ltd: Chichester.

Zheng, J. (2009) FRET and its biological application as a molecular ruler, in Handbook of Modern Biophysics, Vol. 3: Biomedical Applications in Biophysics, Humana Press, (in press)

Bykova, E.A. and J. Zheng (2009) Spectra FRET, a fluorescence resonance energy transfer method in live cells, Annual Reviews in Fluorescence 2007, Chapter 4, Springer, (in press)

Zheng, J. (2008) Patch fluorometry, In: Encyclopedia of Life Sciences (ELS), John Wiley & Sons, Ltd: Chichester.

Chen, T.Y., Y.F. Lin, and J. Zheng (2008) Electrophysiological measurements of membrane proteins, in Handbook on Biophysics, volume 1 Chapter 5

Vetter, I., W. Cheng, M. Peiris, B.D. Wyse, S.J. Roberts-Thomson, J. Zheng, G.R. Monteith, P.J. Cabot (2008) Rapid, opioid-sensitive mechanisms involved in transient receptor potential vanilloid 1 sensitization, Journal of Biological Chemistry, 283, 19540-19550

Ying, G., S.S. Liu, S. Qiu, W. Cheng, J. Zheng, J.H. Luo (2007) Fluorescence resonance energy transfer analysis of subunit assembly of the ASIC channel, Biochemical and Biophysical Research Communications, 359, 143-150

Cheng, W., F. Yang, C.L. Takanishi, and J. Zheng (2007) Thermosensitive TRPV Channel Subunits Co-assemble into Heteromeric Channels with Intermediate Conductance and Gating Properties, Journal of General Physiology, 129, 191-207 ** featured on the cover **

Bykova, E.A., X.D. Zhang, T.Y. Chen, and J. Zheng (2006) Large movement in the C terminus of CLC-0 chloride channel during slow gating, Nature Structural and Molecular Biology, 13, 1115-1119

Jie Zheng (2006) Spectroscopy-based quantitative fluorescence resonance energy transfer analysis, Methods in Molecular Biology, 337, 65-78

Jie Zheng (2006) Patch fluorometry: shedding new light on ion channels, Physiology, 21, 6-12

Christina L. Takanishi, Ekaterina A. Bykova, Wei Cheng, and Jie Zheng (2006) Quantification of GFP-based FRET in transfected cells, Brain Research, 1091(1), 132-139

Jie Zheng, and William N. Zagotta (2004) Stoichiometry and assembly of olfactory cyclic nucleotide-gated channels, Neuron, 42, 411-421

Zheng, J., and W.N. Zagotta (2003) Patch-clamp fluorometry recording of conformational rearrangements of ion channels, Science's STKE, pl7

Zheng, J., M.D. Varnum, and W.N. Zagotta (2003) Disruption of an intersubunit interaction underlies Ca2+-calmodulin modulation of cyclic nucleotide-gated channels, Journal of Neuroscience, 23(22), 8167-8175

Zheng, J., M.C. Trudeau, and W.N. Zagotta (2002) Rod cyclic nucleotide-gated channels have a stoichiometry of three CNGA1 subunits and one CNGB1 subunit, Neuron, 36, 891-896 ** Commentary in Nature Review Neuroscience and in Neuron **

Zheng, J., L. Venkataramanan, and F.J. Sigworth (2001) Hidden Markov model analysis of intermediate gating steps associated with the pore gate of Shaker potassium channels, Journal of General Physiology, 118, 547-562

Zheng, J., and W.N. Zagotta (2000) Gating rearrangements in cyclic nucleotide-gated channels revealed by patch-clamp fluorometry, Neuron, 28, 369-374

Zheng, J., and F.J. Sigworth (1998) Intermediate conductances during deactivation of heteromultimeric Shaker potassium channels, Journal of General Physiology, 112, 457-474

Zheng, J., and F.J. Sigworth (1997) Selectivity changes during activation of mutant Shaker potassium channels, Journal of General Physiology, 110, 101-117 ** featured on the cover **

Mathur, R., J. Zheng, Y. Yan, and F.J. Sigworth (1997) Role of the S3-S4 linker in Shaker potassium channel activation, Journal of General Physiology, 109, 191-200

Zhang, R.J., D.J. Zou, and J. Zheng (1995) Correlation of receptive field position of mechanosensory neurons and the strength of their connections to AP neurons in the CNS of the leech (Whitmania pigra), Invertebrate Neuroscience, 1, 249-254

Zhang, R.J., D.J. Zou, J. Zheng, and W.H. Xiong (1992) Positional discrimination of the AP neuron synapses in the leech Whitmania pigra, Science in China, Series B, 12, 1287-1294

Liu, K.Q., J. Zheng, Y.Q. Lu, Y.H. Zhang, and F. Liu (1991) Automatic analysis of single-channel records, Acta Biophysica Sinica, 7(3), 346-351

Research Interests:
Ion channels form the basis of electrical excitability of neurons and muscle cells. In response to specific electrical or chemical stimuli, these membrane proteins open a pathway across the cell membrane for selected ions, causing changes in membrane potential or intracellular levels of calcium. Activation of an ion channel is under extremely precise control that allows highly specialized processes, e.g., photoreceptor cells to detect the presence of a single photon. Research in my laboratory focuses on the protein structures critical for channel organization, activation, and modulation. The moving parts of the channel are labeled with fluorophores that serve as a molecular sensor for local conformational rearrangements. Recording the fluorescence emission let us directly observe channel structural changes in real time under physiological conditions. The same population of channels is simultaneously monitored with patch-clamp recordings so that we can correlate structure changes to the channel's function states. In particular, we use Fluorescence Resonance Energy Transfer (FRET) to measure atomic distances between channel structures.

Laboratory Personnel:
Current Member: - Fan Yang, Yuanyuan (Iris) Cui

Previous lab members: - Chris Takanishi, Katya Bykova, Wei Cheng, Ping Liang

Courses Taught:
MCP 210A Advanced Physiology - Term(s): Fall
HPH 400 Physiology - Term(s): Fall
PTX 202 Principles of Pharmacology and Toxicology - Term(s): Winter