Faculty Profile
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Frederic Chedin
Assistant Professor Molecular and Cellular Biology (College of Biological Sciences) 332 BRIGGS HALL Office 530-752-1800 Lab 530-752-8224 flchedin@ucdavis.edu http://www.mcb.ucdavis.edu/faculty-labs/chedin/ |
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Degrees:
1995 - PhD - University of Paris - France - DNA recombination in Bacteria
1991 - MS - University of Paris - France - DNA repair and recombination
Awards:
Excellence in Research PhD award - INRA, 1991-1995
NATO post doctoral fellowship - 1996-1998
Basil O'Connor Starter Research Scholar Award - March of dimes foundation - 2005-2007
Department and Center Affiliations:
Department of Molecular and Cellular Biology
Professional Societies:
DNA Methylation Society
Grad Group Affiliations and Specialties:
Biochemistry and Molecular Biology
Cell and Developmental Biology
Genetics
Publications:
Kareta M. S., Botello Z., Ennis J. E., Chou C. and Chedin F. (2006) Reconstitution and mechanism of the stimulation of de novo methylation by human DNMT3L. Journal of Biological Chemistry. 281:25893-25902.
Chen Z., Mann J.R., Hsieh C.L., Riggs A.D. and Chedin F. (2005). Physical and functional interactions between the human DNMT3L protein and members of the de novo methyltransferase family. Journal of Cellular Biochemistry. 95: 902-917
Yu K., Chedin F., Hsieh C.L., Wilson T.E. and M.R. Lieber. (2003). R-loops at immunoglobulin class switch regions in the chromosomes of stimulated B cells. Nature Immunology. 4: 442-451.
Chedin F., Lieber M.R. and C.L. Hsieh. (2002) The DNA methyltransferase-like protein DNMT3L stimulates de novo methylation by Dnmt3a. Proceedings of the National Academy of Sciences USA. 99: 16916-16921.
Research Interests:
Molecular mechanisms of epigenetic gene regulation. Mammalian DNA methyltransferases catalyze the addition of methyl groups to the carbon 5 of the cytosine ring located within CG dinucleotides. DNA methylation, particularly when applied to CG-rich promoter sequences, has been shown to silence gene expression in a heritable manner. The transcriptional silencing associated with 5-methylcytosine is required for fundamental physiological processes such as embryonic development, protection against intragenomic parasites, X-inactivation and genomic imprinting. In addition, aberrant promoter methylation and inappropriate silencing of tumor suppressor genes has recently emerged as a major cause leading to cancer. We are using a combination of approaches (biochemistry, structural biology, mammalian cell culture) to study the function of DNA methyltransferases in mammalian cells.
Laboratory Personnel:
Chedin Lab - 341 Briggs Hall - Mike Kareta, BMB graduate student / Bethany Wienholz, Genetics graduate student / Amir Moarefi, CDB graduate student / Catherine Gordon, Genetics graduate student / Duncan Johnston, Technician / Undergrads: Alex Arzeno, Yoong Wearm Lim, Madeline Nguyen, Nicole Sadler
http://www.mcb.ucdavis.edu/faculty-labs/chedin/index.htm
Courses Taught:
MCB 162 Human Genetics - Term(s): Fall
BMB 290 Mammalian Epigenetics - Term(s): Spring
BMB 220L Laboratory rotation for BMB / CDB - Term(s): Fall,Winter
